Mouse Anti-PG IgG Elisa Kit 96T
- Product name: Mouse IgG ELISA Kit
- Detection method: Colorimetric
- Example type: Serum, cell culture media, mouse IgG
- Test type: Sandwich (quantitative)
- Sensitivity: 1 ng / ml
- Test time: 3 h 15 min
- Trial duration: Standard multi-step test
- Reacts with: Mouse, Rat
- Does not react with: Goat, Cow
ab151276, a mouse IgG ELISA (enzyme-linked immunosorbent assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IgG in serum, plasma, and mouse cell culture supernatant. This assay employs a mouse IgG-specific antibody coated on plate strips. Standards and samples are pipetted into the wells and the IgG present in the sample is bound to the wells by the immobilized antibody.
Wells are washed and HRP-conjugated anti-mouse IgG detector antibody is added. After washing the unbound detector antibody, a TMB substrate solution is added to the wells and color develops in proportion to the amount of bound IgG. The developing blue color is measured at 600 nm. Optionally, the reaction can be stopped with the stop solution that changes the color from blue to yellow and the intensity can be measured at 450 nm.
There are five classes of mammalian immunoglobulins: IgA, IgD, IgE, IgM, and IgG. IgG is the most abundant immunoglobulin and is distributed equally in blood and tissues. In mice, the IgG class is divided into four subclasses: IgG1, IgG2a / IgG2c (strain-specific), IgG2b, and IgG3. The general structure of immunoglobulin is composed of four polypeptide chains, two heavy and two light chains linked together by disulfide bonds, creating a tetrameric quaternary structure. The resulting tetramer creates two identical halves that together form a Y-like structure.
While amino-terminal portions exhibiting highly variable amino acid composition are involved in antigen binding, constant C-terminal parts are involved in binding. complement, placental passage, and cell membrane attachment. IgG is involved in response to a foreign antigen. The presence of IgG generally signifies a mature antibody response. IgG has a molecular weight of approximately 150 kDa, can bind to many pathogens, and also plays an important role in antibody-dependent cell-mediated cytotoxicity. Typically, mouse serum and plasma samples contain approximately 7 to 10 mg/ml of IgG.
Store at + 4 ° C. Consult protocols.
Cell location: Secreted
- Region C of the Ig gamma 1 chain
- Region C of the Ig gamma 2 chain
- Ig gamma chain 3 C region