• July 3, 2021

General Cyclic Diguanylate ELISA K

  • Product name: Cyclic Diguanylate (CdiGMP), ELISA Kit
  • Full Product Name: General Cyclic Diguanilate ELISA Kit

  • Product synonym names: Cyclic Diguanylate; CdiGMP
  • Product gene name: CdiGMP ELISA kit
  • Species reactivity: general

Specificity

This assay recognizes recombinant and natural general CdiGMP. No significant interference or cross-reactivity was observed.

Samples

Serum, plasma, tissue homogenates, Cell culture supernatants, and other biological fluids

Test type: Quantitative competitive

Detection range: 0.78 ng / ml – 50 ng / ml

Preparation and storage: Store at 4 degrees C

Product note

Information selected from the online data sheet is extracted from bioinformatics databases, occasionally resulting in ambiguous or irrelevant product information. It is the customer’s responsibility to review, verify and evaluate the information to make sure it matches their requirements before purchasing the kit. The assays in our ELISA kit are dynamic research tools and can sometimes be updated and improved.

If the format of this assay is important to you, please request the current manual or contact our technical support team with a pre-sale consultation before placing an order. We will confirm the current details of the trial. We cannot guarantee that the sample manual posted online is the most current manual, it is intended to serve as an example only. See the instructions for use provided with the test kit for precise details.

Other notes

Occasionally, small volumes of the CdiGMP ELISA kit vials may become trapped in the product vial seal during shipping and storage. If necessary, briefly centrifuge the vial in a benchtop centrifuge to dislodge any liquid on the lid of the container. Some products may need to be shipped with dry ice and an additional fee may apply for dry ice.

Search terms for purchasing CdiGMP

MBS9425257 is a ready-to-use, strip or whole plate, microwell ELISA (enzyme-linked immunosorbent assay) kit for testing the presence of cyclic diguanylate ELISA kit (CdiGMP) target analytes in biological samples. The concentration gradients of the kit standards or positive controls provide a theoretical detection range of the kit in biological research samples containing CdiGMP.

The analytical biochemical ELISA technique of the MBS9425257 kit is based on CdiGMP antibody-CdiGMP antigen interactions (immunoadsorption) and an HRP colorimetric detection system to detect CdiGMP antigen targets in samples. The ELISA Kit is designed to detect native, non-recombinant CdiGMP. Appropriate sample types may include secretions and undiluted body fluids and/or tissue homogenates. Quality control trials evaluating reproducibility identified the intra-assay CV (%) and inter-assay CV (%).

Related Product Information for CdiGMP ELISA kit

Intended Uses: This immunoassay kit allows for the quantitative in vitro determination of general concentrations of CdiGMP in serum, plasma, tissue homogenates, cell culture supernatants, and other biological fluids.

Principle of the assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific for CdiGMP; During the reaction, CdiGMP in the sample or standard competes with a fixed amount of biotin-labeled CdiGMP for sites on a pre-coated antibody specific for CdiGMP. Excess conjugate and unbound samples or standards are washed from the plate.

Avidin conjugated to horseradish peroxidase (HRP) is then added to each well of the microplate and incubated. A TMB Substrate Solution is then added to each well. The enzyme-substrate reaction is terminated by adding a sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of cGMP in the samples is determined by comparing the O.D. of the samples to the standard curve.

Precautions

All MyBioSource products are for scientific laboratory research purposes and not for diagnostic, therapeutic, prophylactic, or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Product purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order when we reasonably believe that the intended use will be outside of our acceptable guidelines.

Disclaimer

While every effort has been made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be responsible for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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