Ovo i doustne podawanie probiotycznych
In Ovo i doustne podawanie probiotycznych pałeczek kwasu mlekowego moduluje odpowiedź immunologiczną za pośrednictwem komórek i przeciwciał u nowo wyklutych piskląt
There’s some proof that lactobacilli can strengthen the immune system of chickens. This research evaluated the consequences of in ovo and oral administration of a lactobacilli cocktail on cytokine gene expression, antibody-mediated immune responses, and spleen cellularity in chickens. Lactobacilli have been administered both in ovo at embryonic day 18, orally at days 1, 7, 14, 21, and 28 post-hatches, or a mix of each in ovo and post-hatch inoculation. On day 5 and 10 post-hatch, spleen and bursa of Fabricius have been collected for gene expression and cell composition evaluation.
On days 14 and 21 post-hatch, birds have been immunized with sheep crimson blood cells (SRBC) and keyhole limpet hemocyanin (KLH), and sera have been collected on days 7, 14, and 21 post-primary immunization. Birds that obtained lactobacilli (107 CFU) by way of in ovo adopted by weekly oral administration confirmed a larger immune response by enhancing antibody responses, rising the proportion of CD4+ and CD4+CD25+ T cells within the spleen and upregulating the expression of interferon (IFN)-α, IFN-β, interleukin (IL)-8, IL-13, and IL-18 within the spleen and expression of IFN-γ, IL-2, IL-6, IL-8, IL-12, and IL-18 within the bursa. These findings counsel that pre-and post-hatch administration of lactobacilli can modulate the immune response in newly hatched chickens.
Anti-p16 (mouse monoclonal) |
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| DB253RTU-15 | DB Biotech | 15 ml | EUR 416.4 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Normal Rabbit Serum | RS-15-100ML |
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| RS-15-100ML | Immunology Consultants Laboratory | 100 mL | EUR 420 |
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Description: Normal Rabbit Serum | RS-15-100ML | Immunology Consultants Laboratory Species Reactivity: Rabbit Format: Serum Product Type: Isotype Control Antibody clonality: Polyclonal |
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Mouse ADAM ELISA Kit |
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| EMA0733 | Abclonal | 96Tests | EUR 625.2 |
ADAM Metallopeptidase With Thrombospondin Type 1 Motif 15 (ADAMTS15) Antibody |
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| 20-abx334251 | Abbexa |
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ADAM Metallopeptidase With Thrombospondin Type 1 Motif 15 (ADAMTS15) Antibody |
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| 20-abx212035 | Abbexa |
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ADAM Metallopeptidase With Thrombospondin Type 1 Motif 15 (ADAMTS15) Antibody |
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| 20-abx212386 | Abbexa |
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ADAM Metallopeptidase With Thrombospondin Type 1 Motif 15 (ADAMTS15) Antibody |
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| 20-abx212831 | Abbexa |
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Sulfur 15 ug/g (15 PPM) for AA and ICP 100 grams in no.2 Diesel Fuel |
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| SDFS-15-Y | Scientific Laboratory Supplies | 100ML | EUR 102.6 |
Polyclonal Goat anti-GST α-form |
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| GST-ANTI-1 | Detroit R&D | 50 uL | EUR 336 |
Polyclonal Goat anti-GST μ-form |
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| GST-ANTI-2 | Detroit R&D | 50 uL | EUR 336 |
Polyclonal Goat anti-GST p-form |
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| GST-ANTI-3 | Detroit R&D | 50 uL | EUR 336 |
UltraPolymer Goat Anti-Mouse IgG (H&L) HRP |
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| A1275-15 | Biovision | each | EUR 385.2 |
ADAM Metallopeptidase With Thrombospondin Type 1 Motif 15 (ADAMTS15) Antibody (HRP) |
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| 20-abx334527 | Abbexa |
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ADAM Metallopeptidase With Thrombospondin Type 1 Motif 15 (ADAMTS15) Antibody (FITC) |
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| 20-abx334528 | Abbexa |
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ADAM Metallopeptidase With Thrombospondin Type 1 Motif 15 (ADAMTS15) Antibody (Biotin) |
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| 20-abx334529 | Abbexa |
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DiagNano Gold Nanoparticle Medium Covalent Conjugation Kit, 15 nm |
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| GCK-M-15 | Creative Diagnostics | 1 kit | EUR 908.4 |
DiagNano Fluorophore Labeled Gold Nanoparticles, 15 nm, Cellular Uptake |
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| GFL-15-CU | Creative Diagnostics | 1mL | EUR 1638 |
UltraPolymer Goat Anti-Rabbit/Mouse IgG (H&L) HRP Cocktail |
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| A1280-15 | Biovision | each | EUR 385.2 |
Anti-iNOS |
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| DB003RTU-15 | DB Biotech | 15 ml | EUR 513.6 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-p53 |
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| DB026RTU-15 | DB Biotech | 15 ml | EUR 405.6 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-CD5 |
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| DB027RTU-15 | DB Biotech | 15 ml | EUR 496.8 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-CD20 |
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| DB041RTU-15 | DB Biotech | 15 ml | EUR 424.8 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-CD45 |
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| DB042RTU-15 | DB Biotech | 15 ml | EUR 405.6 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-CD163 |
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| DB045RTU-15 | DB Biotech | 15 ml | EUR 424.8 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-Melanosome |
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| DB049RTU-15 | DB Biotech | 15 ml | EUR 424.8 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-CD10 |
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| DB057RTU-15 | DB Biotech | 15 ml | EUR 496.8 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-CD117 |
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| DB062RTU-15 | DB Biotech | 15 ml | EUR 405.6 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-CD1a |
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| DB071RTU-15 | DB Biotech | 15 ml | EUR 496.8 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-CD3 |
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| DB082RTU-15 | DB Biotech | 15 ml | EUR 424.8 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-CD8 |
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| DB085RTU-15 | DB Biotech | 15 ml | EUR 496.8 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-EGFR |
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| DB092RTU-15 | DB Biotech | 15 ml | EUR 424.8 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-CD7 |
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| DB114RTU-15 | DB Biotech | 15 ml | EUR 513.6 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-MSH2 |
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| DB115RTU-15 | DB Biotech | 15 ml | EUR 480 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-p63 |
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| DB134RTU-15 | DB Biotech | 15 ml | EUR 416.4 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-CD23 |
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| DB135RTU-15 | DB Biotech | 15 ml | EUR 514.8 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-Desmin |
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| DB148RTU-15 | DB Biotech | 15 ml | EUR 416.4 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-CD34 |
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| DB151RTU-15 | DB Biotech | 15 ml | EUR 416.4 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-p16 |
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| DB152RTU-15 | DB Biotech | 15 ml | EUR 416.4 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-Synaptophysin |
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| DB156RTU-15 | DB Biotech | 15 ml | EUR 424.8 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-Braf |
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| DB159RTU-15 | DB Biotech | 15 ml | EUR 850.8 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-p60 |
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| DB203RTU-15 | DB Biotech | 15 ml | EUR 416.4 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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anti-Calpain 15 |
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| YF-PA24745 | Abfrontier | 50 ul | EUR 400.8 |
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Description: Mouse polyclonal to Calpain 15 |
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anti-Cytokeratin 15 |
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| YF-PA12886 | Abfrontier | 100 ul | EUR 483.6 |
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Description: Rabbit polyclonal to Cytokeratin 15 |
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anti-Cytokeratin 15 |
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| YF-PA12887 | Abfrontier | 100 ug | EUR 483.6 |
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Description: Rabbit polyclonal to Cytokeratin 15 |
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anti-Claudin 15 |
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| YF-PA18066 | Abfrontier | 50 ug | EUR 435.6 |
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Description: Mouse polyclonal to Claudin 15 |
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anti-Claudin 15 |
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| YF-PA18067 | Abfrontier | 100 ug | EUR 483.6 |
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Description: Rabbit polyclonal to Claudin 15 |
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anti-Kallikrein 15 |
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| YF-PA19752 | Abfrontier | 50 ul | EUR 435.6 |
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Description: Mouse polyclonal to Kallikrein 15 |
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1-step Polymer HISTO-STAT; HRP anti Mouse for staining Mouse and Rat antibodies, 250 plus slides |
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| NB312M-15 | Innovex | 15 ml | EUR 512.4 |
ADAM-17 Antibody |
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| 39313-100ul | SAB | 100ul | EUR 468 |
TACE (ADAM-17) |
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| 5-01998 | CHI Scientific | 4 x 1mg | Ask for price |
ADAM 17 Antibody |
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| ABF6361 | Lifescience Market | 100 ug | EUR 525.6 |
ADAM 17 Antibody |
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| AF6361 | Affbiotech | 200ul | EUR 420 |
Anti-b-actin |
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| DB001RTU-15 | DB Biotech | 15 ml | EUR 318 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-Melan A |
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| DB050RTU-15 | DB Biotech | 15 ml | EUR 362.4 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-Cytokeratin 7 |
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| DB051RTU-15 | DB Biotech | 15 ml | EUR 370.8 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-S-100 |
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| DB055RTU-15 | DB Biotech | 15 ml | EUR 424.8 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-Cyclin D1 |
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| DB066RTU-15 | DB Biotech | 15 ml | EUR 496.8 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-Ki-67 |
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| DB070RTU-15 | DB Biotech | 15 ml | EUR 424.8 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-TTF-1 |
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| DB089RTU-15 | DB Biotech | 15 ml | EUR 513.6 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-Cytokeratin 8 |
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| DB098RTU-15 | DB Biotech | 15 ml | EUR 370.8 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-Cytokeratin 14 |
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| DB099RTU-15 | DB Biotech | 15 ml | EUR 362.4 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-Cytokeratin 16 |
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| DB100RTU-15 | DB Biotech | 15 ml | EUR 362.4 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-Cytokeratin 17 |
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| DB101RTU-15 | DB Biotech | 15 ml | EUR 362.4 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-Cytokeratin 18 |
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| DB102RTU-15 | DB Biotech | 15 ml | EUR 318 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-Cytokeratin 19 |
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| DB103RTU-15 | DB Biotech | 15 ml | EUR 318 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-C3d complement |
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| DB106RTU-15 | DB Biotech | 15 ml | EUR 424.8 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-C4d complement |
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| DB107RTU-15 | DB Biotech | 15 ml | EUR 424.8 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-Ki-67 |
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| DB111RTU-15 | DB Biotech | 15 ml | EUR 424.8 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-Cytokeratin 20 |
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| DB119RTU-15 | DB Biotech | 15 ml | EUR 370.8 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-Chromogranin A |
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| DB158RTU-15 | DB Biotech | 15 ml | EUR 424.8 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-Human IgG |
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| DB173RTU-15 | DB Biotech | 15 ml | EUR 426 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-Human IgG |
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| DB174RTU-15 | DB Biotech | 15 ml | EUR 426 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-P504S (AMACR) |
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| DB208RTU-15 | DB Biotech | 15 ml | EUR 408 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Mouse ADAM DEC1(ADAMDEC1) ELISA kit |
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| CSB-EL001297MO-24T | Cusabio | 1 plate of 24 wells | EUR 198 |
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Description: Quantitativesandwich ELISA kit for measuring Mouse ADAM DEC1 (ADAMDEC1) in samples from serum, plasma, tissue homogenates, cell culture supernates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
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Mouse ADAM DEC1(ADAMDEC1) ELISA kit |
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| 1-CSB-EL001297MO | Cusabio |
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Description: Quantitativesandwich ELISA kit for measuring Mouse ADAM DEC1(ADAMDEC1) in samples from serum, plasma, tissue homogenates, cell culture supernates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
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Mouse ADAM DEC1(ADAMDEC1) ELISA kit |
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| E03A1245-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A sandwich ELISA for quantitative measurement of Mouse ADAM DEC1(ADAMDEC1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse ADAM DEC1(ADAMDEC1) ELISA kit |
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| E03A1245-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A sandwich ELISA for quantitative measurement of Mouse ADAM DEC1(ADAMDEC1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse ADAM DEC1(ADAMDEC1) ELISA kit |
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| E03A1245-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A sandwich ELISA for quantitative measurement of Mouse ADAM DEC1(ADAMDEC1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse ADAM DEC1, Adamdec1 ELISA KIT |
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| ELI-11570m | Lifescience Market | 96 Tests | EUR 1038 |
Anti-ER (Estrogen Receptor) |
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| DB053RTU-15 | DB Biotech | 15 ml | EUR 487.2 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-PR (Progesterone Receptor) |
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| DB059RTU-15 | DB Biotech | 15 ml | EUR 513.6 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-EBV/LMP-1 |
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| DB060RTU-15 | DB Biotech | 15 ml | EUR 513.6 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-EBV/LMP-1 |
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| DB061RTU-15 | DB Biotech | 15 ml | EUR 513.6 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-Cytokeratin 5/6 |
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| DB097RTU-15 | DB Biotech | 15 ml | EUR 424.8 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-PR (Progesterone Receptor) |
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| DB108RTU-15 | DB Biotech | 15 ml | EUR 513.6 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-PR (Progesterone Receptor) |
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| DB109RTU-15 | DB Biotech | 15 ml | EUR 513.6 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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TruStrip Sample Transfer Strips, 15-ul, 50/Pk (without sample tracking dye) |
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| STS-15-50 | Alpha Diagnostics | 1 Pk | Ask for price |
TruStrip Sample Transfer Strips, 15-ul, 50/Pk (with sample tracking dye) |
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| STSD-15-50 | Alpha Diagnostics | 1 Pk | Ask for price |
Anti-Human Kappa Light Chain |
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| DB037RTU-15 | DB Biotech | 15 ml | EUR 424.8 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-Human Kappa Light Chain |
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| DB038RTU-15 | DB Biotech | 15 ml | EUR 424.8 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-Human Lambda Light Chain |
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| DB039RTU-15 | DB Biotech | 15 ml | EUR 424.8 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-PLAP (Placental Alkaline Phosphatase) |
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| DB047RTU-15 | DB Biotech | 15 ml | EUR 318 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-EMA (CD227, Mucin-1) |
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| DB048RTU-15 | DB Biotech | 15 ml | EUR 318 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-Alpha Smooth Muscle Actin |
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| DB147RTU-15 | DB Biotech | 15 ml | EUR 416.4 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-p16 (mouse monoclonal) |
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| DB-253-RTU-15 | DB Biotech | 15 ml | EUR 416.4 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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1-step Polymer HISTO-STAT; HRP anti Mouse Adsorbed (Secondary Reagent Component) for staining Rat antibody-on-Mouse tissues, 250 plus slides |
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| NB312MRA-15 | Innovex | 15 ml | EUR 651.6 |
Mouse Interleukin-15 (IL-15) Antibody |
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| 32200-05111 | AssayPro | 150 ug | EUR 313.2 |
Anti-MMP-15 Antibody |
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| A06396 | BosterBio | 100ul | EUR 476.4 |
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Description: Rabbit Polyclonal MMP-15 Antibody. Validated in WB and tested in Human, Mouse. |
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Anti-Cytokeratin 15 Antibody |
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| A06791 | BosterBio | 100ul | EUR 476.4 |
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Description: Rabbit Polyclonal Antibody for Cytokeratin 15 Antibody (KRT15) detection.tested for WB in Human, Mouse, Rat, Monkey. |
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Anti-DHHC-15 Antibody |
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| A12099 | BosterBio | 100ul | EUR 476.4 |
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Description: Rabbit Polyclonal Antibody for DHHC-15 Antibody (ZDHHC15) detection. Tested with WB in Human. |
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Anti-GDF-15 Antibody |
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| A2038-100 | Biovision | 100 µl | EUR 502.8 |
Anti-Cytokeratin 15 antibody |
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| PAab02198 | Lifescience Market | 100 ug | EUR 426 |
anti-CDC2 (Ab-15) |
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| LF-PA20070 | Abfrontier | 100 ul | EUR 400.8 |
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Description: Rabbit polyclonal to CDC2 |
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anti-HSP27 (Ab-15) |
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| LF-PA20219 | Abfrontier | 100 ul | EUR 400.8 |
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Description: Rabbit polyclonal to HSP27 |
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anti-p53 (Ab-15) |
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| LF-PA20358 | Abfrontier | 100 ul | EUR 400.8 |
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Description: Rabbit polyclonal to p53 |
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anti- Cytokeratin 15 antibody |
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| FNab02198 | FN Test | 100µg | EUR 606.3 |
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Description: Antibody raised against Cytokeratin 15 |
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anti- Cytokeratin 15 antibody |
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| FNab02199 | FN Test | 100µg | EUR 658.5 |
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Description: Antibody raised against Cytokeratin 15 |
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Anti-Cytokeratin 15 antibody |
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| STJ92628 | St John's Laboratory | 200 µl | EUR 236.4 |
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Description: Rabbit polyclonal to Cytokeratin 15. |
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Anti-DHHC-15 antibody |
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| STJ92707 | St John's Laboratory | 200 µl | EUR 236.4 |
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Description: Rabbit polyclonal to DHHC-15. |
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Anti-GDF-15 antibody |
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| STJ93253 | St John's Laboratory | 200 µl | EUR 236.4 |
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Description: Rabbit polyclonal to GDF-15. |
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Przeciwciało anty-Rac1-GTP i wykrywanie aktywnego Rac1: narzędzie z podstawową wadą
Rac1 is a member of the Rho GTPase household and is concerned in lots of mobile processes, notably the formation of actin-rich membrane protrusions, corresponding to lamellipodia and ruffles. With such a broadly studied protein, it’s important that the analysis neighborhood has dependable instruments for detecting Rac1 activation each in mobile fashions and tissues. Utilizing a collection of most cancers mobile fashions, we not too long ago demonstrated {that a} broadly used antibody for visualizing lively Rac1 (Rac1-GTP) doesn’t acknowledge Rac1 however as a substitute acknowledges vimentin filaments (Baker MJ, J. Biol. Chem. 295:13698-13710, 2020).
We imagine that this software has misled the sector and impose on the GTPase analysis neighborhood the necessity to validate revealed outcomes utilizing this antibody in addition to to proceed the event of latest assets to visualise endogenous lively Rac1.
Wpływ substytucji N501Y w SARS-CoV-2 Spike na neutralizację przeciwciał monoklonalnych ukierunkowanych na różne epitopy
The emergence and fast unfold of the B.1.1.7 lineage (VOC-202012/01) SARS-CoV-2 variant has aroused international concern. The N501Y substitution is the one mutation within the interface between the RBD of B.1.1.7 and ACE2, elevating considerations that its recognition by neutralizing antibodies could also be affected. Right here, we assessed the neutralizing exercise and binding affinity of a panel of 12 monoclonal antibodies towards the wild kind and N501Y mutant SARS-CoV-2 pseudovirus and RBD protein, respectively.
We discovered that the neutralization exercise and binding affinity of most detected antibodies (10 out of 12) have been unaffected, though the N501Y substitution decreased the neutralizing and binding actions of CB6 and elevated that of BD-23. These findings may very well be of worth within the improvement of therapeutic antibodies.
Izolacja ludzkiego przeciwciała monoklonalnego specyficznego dla domeny wiążącej receptor SARS-CoV-2 przy użyciu konkurencyjnej strategii biopanningu
- has triggered greater than 200 000 deaths, however no vaccine or therapeutic monoclonal antibody is at present obtainable. SARS-CoV-2 depends on its spike protein, particularly the receptor-binding area (RBD), to bind human cell receptor angiotensin-converting enzyme 2 (ACE2) for viral entry, and thus concentrating on RBD holds the promise for stopping SARS-CoV-2 an infection.
- On this work, a aggressive biopanning technique of a phage show antibody library was utilized to display blocking antibodies towards RBD. Excessive-affinity antibodies have been enriched after the primary spherical utilizing a regular panning course of by which RBD-His was immobilized as a bait. On the subsequent two rounds, immobilized ACE2-Fc and free RBD-His have been blended with the enriched phage antibodies. Antibodies binding to RBD at epitopes completely different from ACE2-binding web site have been captured by the immobilized ACE2-Fc, forming a “sandwich” advanced.
- Solely antibodies competed with ACE2 can bind to the free RBD-His within the supernatant and be subsequently separated by the nickel-nitrilotriacetic acid magnetic beads. rRBD-15 from the aggressive biopanning of our artificial antibody library, Lib AB1, was produced because the full-length IgG1 format.
- It was proved to competitively block the binding of RBD to ACE2 and potently inhibit SARS-CoV-2 pseudovirus an infection with IC50 values of 12 nM. However, rRBD-16 from the usual biopanning can solely bind to RBD in vitro, however not have the blocking or neutralization exercise. Our technique can effectively isolate the blocking antibodies of RBD, and it might velocity up the invention of neutralizing antibodies towards SARS-CoV-2.
10-letnie badanie dotyczące wskaźników i miana przeciwciał w surowicy w kierunku odry i różyczki u pracowników służby zdrowia; badanie obserwacyjne w japońskim szpitalu uniwersyteckim
Background: We evaluated the impact of the two-dose vaccination technique, which has been a broadly adopted as childhood routine schedule worldwide to amass herd immunity, on healthcare staff (HCWs) in Japan.
Strategies: Between 2010 and 2019, antibody titers for measles and rubella have been measured yearly amongst newly employed HCWs at Osaka College Hospital, Japan, utilizing Enzygnost® assays (Siemens Healthcare Diagnostics Co. Ltd., Marburg, Germany). The info have been categorized by age to match the antibody positivity charges and antibody titers amongst no-vaccine, single-dose, and two-dose teams.
Outcomes: Over the 10-year interval, the annual antibody positivity charges for measles and rubella have been 84.0%-95.3% and 90.0%-94.5%, respectively, with none specific pattern. The antibody titers for measles (median [interquartile range]: 8.4 [3.9, 20] vs. 6.1 [3.5, 12]) and rubella (11 [5.5, 20] vs. 6 [3.7, 11]) have been statistically decrease (p < 0.001) within the two-dose era than within the single-dose era.
Dialogue: A shift from single-dose to two-dose vaccination didn’t yield a rise in antibody positivity charges for each measles and rubella amongst HCWs. Notably, antibody titers have been considerably decrease within the two-dose era.
Conclusion: Regardless of a number of limitations, our information suggests a paradoxical vulnerability in younger HCWs who obtained the two-dose vaccination in a view of sero-positivity charges.
