• April 30, 2021
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Przeciwciała przeciwko dekarboksylazie

Przeciwciała przeciwko dekarboksylazie glutaminianowej w surowicy a zaburzenia neurologiczne: kiedy podejrzewać ich związek?

Goals: To discover completely different neurological manifestations with suspicion of being related to serum glutamate decarboxylase antibodies (GAD-Abs) in order to raised characterize anti-GAD neurological syndromes.

Strategies: Observational retrospective research together with all sufferers for whom GAD65-Abs titers in serum have been requested by the Neurology Division at La Paz College Hospital between 2015 and 2019. GAD-Abs have been measured by ELISA. Demographic knowledge, neurological signs, comorbidity with diabetes mellitus (DM) or with one other autoimmune illness, and GAD-Abs titers have been studied. Stiff-person syndrome, ataxia, encephalitis, and epilepsy have been thought of typical anti-GAD neurological syndromes and have been in comparison with different atypical manifestations.

Outcomes: A complete of 173 sufferers (51.7% males, imply age 51.62) have been included. A progressive enhance in requests of serum GAD-Abs has occurred over the past 5 years, particularly in sufferers with atypical neurological manifestations. GAD-Abs have been discovered within the serum of 22 sufferers (12.7%); of these, 15 (68.18%) suffered a typical anti-GAD syndrome. Presence of DM or one other organ-specific autoimmune illness was predictive of GAD-AB seropositivity (p < 0.001). 6.6% of requested sufferers with an atypical syndrome had GAD-Abs, however serum ranges have been considerably decrease than these present in sufferers with a typical syndrome (706.67 vs 1430.23 UI/mL; Mann-Whitney U, p = 0.034), and have been lastly identified with one other neurological illness.

Conclusion: Serum GAD-Abs have been sometimes present in sufferers with scientific phenotypes aside from these classically described as anti-GAD issues, and with very low titers. In typical anti-GAD syndromes, there’s a excessive comorbidity with DM and with different autoimmune ailments, and excessive serum GAD-Abs ranges are normally current.

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Podwójne dodatnie wyniki przeciwciał przeciwko β -glikoproteinie I domenie I i przeciw fosfatydyloserynie / protrombinie wzmacniają zarówno zakrzepicę, jak i dodatni wynik przeciwciał anty-ADAMTS13

  • Though a number of antiphospholipid syndrome (APS) happens with acquired thrombotic thrombocytopenic purpura (TTP), the connection between antiphospholipid antibodies (aPL) and anti-ADAMTS13 (anti-a disintegrin and metalloprotease with thrombospondin kind 1 motif, member 13) antibody stays unsure. We investigated the connection between high-risk thrombotic aPL and anti-ADAMTS13 antibody. 2 hundred and thirty-seven sufferers with optimistic lupus anticoagulant and/or anticardiolipin antibody have been included. Anti-β2GPI (anti-β2-glycoprotein I), anti-β2GPIdI (anti-β2-glycoprotein I area I), anti-PS/PT (anti-phosphatidylserine and prothrombin), ADAMTS13 exercise, and anti-ADAMTS13 antibody have been measured. Double positivity of anti-β2GPI and anti-PS/PT elevated thrombotic threat greater than three-fold and confirmed elevated positivity of anti-ADAMTS13 antibody compared with the double adverse group.
  • Double positivity of anti-β2GPIdI and anti-PS/PT introduced each results much more. Within the linear regression evaluation, double positivity of anti-β2GPI and anti-PS/PT independently affected the anti-ADAMTS13 antibody stage (β = 1.982, P = 0.042). Our outcomes revealed that double positivity of anti-β2GPI or anti-β2GPIdI and anti-PS/PT elevated not solely thrombotic threat but in addition the positivity of anti-ADAMTS13 antibody, particularly indicating anti-β2GPIdI confirmed the next synergistic impact with anti-PS/PT. We advise a doable affiliation of anti-ADAMTS13 antibody with a excessive thrombotic threat of APS.
  • Double positivity of anti-β2GPI (anti-β2-glycoprotein I) and anti-PS/PT (anti-phosphatidylserine and prothrombin) antibodies enhanced not solely thrombotic threat but in addition positivity of anti-ADAMTS13 (anti-a disintegrin and metalloprotease with thrombospondin kind 1 motif, member 13) antibody. Moreover, double positivity of anti-β2GPIdI (anti-β2-glycoprotein I area I) mixed with anti-PS/PT much more elevated each thrombosis and positivity of anti-ADAMTS13 antibody. Double positivity of β2GPI and anti-PS/PT was discovered as an independently vital contributing issue to anti-ADAMTS13 antibody stage. We advise the affiliation between anti-ADAMTS13 antibody and the pathophysiology of antiphospholipid syndrome, which needs to be additional evaluated.

Strukturalny wpływ glikodendrymerów rekrutujących przeciwciała (ARG) na cytotoksyczność przeciwnowotworową 

The recruitment of endogenous antibodies towards most cancers cells has turn into a dependable antitumoral immunotherapeutic various over the past decade. The covalent attachment of antibody and tumor binding modules (ABM and TBM) inside a single, well-defined artificial molecule was certainly demonstrated to advertise the formation of an interacting ternary complicated between each the antibodies and the focused cell, which normally leads to the simultaneous immune-mediated mobile destruction. In a preliminary research, we now have described the first Antibody Recruiting Glycodendrimers (ARGs), combining cRGD as ligands for the αVβ3-expressing melanoma cell line M21 and Rha as ligand for pure IgM, and demonstrated that multivalency is an important requirement to type this complicated.

Within the current research, we synthesized a brand new sequence of ARGs composed of ABMs, i.e. self-condensed rhamnosylated cyclopeptide and polylysine dendrimer, which have been conjugated to the TBM with or with out spacer. Movement cytometry and confocal microscopy experiments with human serum and completely different cell traces revealed that the ABM geometry considerably influences the ternary complicated formation in M21, whereas no vital binding happens in BT 549 having low integrin expression.

As well as, we exhibit with a mobile viability assay that ARGs induce excessive stage of cytotoxicity towards M21 which can be in shut correlation with the ABM construction. Specifically, we now have proven that ARG combining cyclopeptide core and branches, with or with out spacer, induce 40-57% of selective cytotoxicity towards M21 cells within the presence of human serum because the distinctive supply of immunity effectors. Lastly, we additionally spotlight that the spacer between ABM and TBM permits a rise of the immune-mediate cytotoxicity even with ABM of decrease valency.

ICELISA na bazie przeciwciał monoklonalnych do badania przesiewowego diazynonu w próbkach warzyw 

Diazinon (DAZ) is an organophosphorus pesticide (OP), which is often used to forestall and management dangerous pests that endanger agricultural merchandise. On this research, we developed a novel heterology coating technique for the immunoassay of DAZ. The DAZ coating hapten could be straight conjugated to the provider protein with out requiring a spacer arm. This proposed hapten coating technique is time-saving and considerably improves the sensitivity of the immunoassay because of the lack of a spacer arm. The as-synthesized coating antigen was used to display the monoclonal antibody (mAb).

Lastly, the developed oblique aggressive enzyme-linked immunoassay (icELISA) confirmed IC50 and restrict of detection (LOD) values of 0.58 ng mL-1 and eight pg mL-1, respectively. This methodology exhibited negligible cross-reactivity in direction of different analogues, and the recoveries of samples (cucumber, cabbage, and lettuce) ranged from 92.6% to 125.4%, with coefficients of variance (CV) beneath 12%. Good correlation between icELISA and high-performance liquid chromatography mass spectrometry (HPLC-MS/MS) was obtained. The proposed icELISA was a really perfect device for monitoring DAZ residues in meals samples.

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