• July 3, 2021

RABBIT ANTI-TYROSINE HYDROXYLASE

  • Biological source: Rabbit
  • Quality level: 100
  • Antibody product type: primary antibodies

  • Clone: polyclonal
  • Purified by: affinity chromatography
  • Species reactivity: Mouse, mollusk, feline, squid, human, ferret, rat, Aplysia (marine mollusk), Drosophila

Application(s)

  • ELISA: adequate
  • immunofluorescence: adequate
  • immunohistochemistry (formalin-fixed, paraffin-embedded sections): adequate
  • immunoprecipitation (IP): adequate
  • Western blot: adequate

NCBI access no: NM_012740.3

UniProt access no: P04177

General description

Tyrosine hydroxylase (also known as tyrosine 3-hydroxylase and tyrosine 3-monooxygenase) plays an important role in the physiology of adrenergic neurons. It is the first and limiting enzyme involved in the biosynthesis of the catecholamines dopamine and norepinephrine from tyrosine. TH is therefore a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified that result from alternative splicing.

Specificity

The antibody is expected to react with most mammalian and many non-mammalian species. This antibody has been reported not to work in paraffin-embedded human tissue. Tyrosine hydroxylase. By Western Blot, AB152 selectively labels a single band at approximately 62 kDa (reduced) corresponding to tyrosine hydroxylase. The antibody is expected to react with most mammalian and many non-mammalian species. This antibody has been reported not to work in paraffin-embedded human tissue.

Immunogen

Rat pheochromocytoma denatured tyrosine hydroxylase (denatured by sodium dodecyl sulfate).

Request

The anti-tyrosine hydroxylase antibody detects the level of TH and has been published and validated for use in ELISA, IF, IH, IH (P), IP, and WB.

Immunohistochemistry:

A previous batch of this antibody was used at a 1: 100-1: 1000 dilution, 4 ° C, for 1-3 days. Stains fresh-frozen and paraffin-embedded tissue samples using indirect immunofluorescence and immunoperoxidase.

Immunofluorescence:

A previous batch of this antibody was used at a dilution of 1: 100-1: 1,000.

Immunoprecipitation:

An older batch of this antibody was used in immunoprecipitation.

ELISA:

  • An older batch of this antibody was used in ELISA.
  • Optimal dilutions must be determined by the end-user.

Quality

Routinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis:

A 1: 1000 dilution of this lot detected tyrosine hydroxylase in 10 µg of PC12 lysates.

Destination description: 62 kDa

Physical form

  • Affinity Purified
  • Buffer purified rabbit polyclonal containing liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl (pH 7.5), 100 ug / ml BSA and 50% glycerol.

Storage and stability

Keep frozen between -20ºC and -70ºC in undiluted aliquots up to 6 months after the date of receipt. Avoid repeated freeze/thaw cycles. Do not store it in a freezer that defrosts automatically.

Legal information

CHEMICAL is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product (s), our products are intended for research use only and are not to be used for any other purpose, including but not limited to commercial uses. unauthorized, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses, or any type of consumption or application to humans or animals.

Leave a Reply

Your email address will not be published. Required fields are marked *